The cart's Podcast

T cell infiltration

T cell infiltration assays ensure the functional T cells are located in tumor microenvironment to display humoral or cellular immunity both in vitro and in vivo.
 

in vivo animal testing

CAR-T cell preclinical in vivo animal testing performed in xenograft murine models derived by well-defined cell lines or primary human tumor cells. Immunodeficient mice, lacking of effective innate immunity for the absence of B cells, T cells, or NK cells, are the optimal animal models for engraftment of tumor cells and evaluation of CAR-T cells.

Electroporation transfer

Electroporation transfer is emerged as a powerful tool for the genetic modification of diverse cell types based on the transient disruption of cell membrane via exposure to an electric field, which allows charged molecules to enter the cell. 

chimeric antigen receptor t cell therapy

Notably, several CAR-T libraries have been generated not only for blood cancer antigen CD19, but also for solid tumors against different antigens, such as Her2, Her3, EGFR, FGFR1, VEGFR, etc. The selected stable clones can be used in clinic trials immediately, thus making this technology more powerful and attractive in chimeric antigen receptor t cell therapy. 

CAR T target

Specifically, a cancer biomarker of the CAR-T provides the most prominent signal of cancer cells for distinguishing from normal cells and the most effective CAR T target for immune recognition and destruction. 

IgG Fab

IgG Fab (also tandem Fab-Fc in the construct) is a format of appended IgGs, the IgG scaffold with Fab fragment in the N terminus.

Fab-arm exchange

The controlled Fab-arm exchange technique platform enables to develop stable IgG-like BsAbs.

DuoBody

Based on the previous studies, the strategies used to overcome the heave chain-pairing problem of BsIgGs include Knob-into-holes (KIH), DuoBody, Azymetric, Charge pair, HA-TH, SEEDbody, differential protein A affinity, etc.

covx body

A bispecific CovX Body contains two different pharmacophores, both of which covalently bound to deep in the hydrophobic binding pockets on each of the two Fab arms of the scaffold antibody.

Bispecific

Bispecific antibodies have the ability to bind to two different epitopes on the same or on different antigens simultaneously. Different domains of BsAbs can be fused by DNA engineering which is also the prevalent method for producing BsAbs.